VDI 4331 Blatt 2
Monitoring the effects of genetically modified organisms (GMOs) - Method for extracting nucleic acids from soil for the analysis of microbial communities and detection of transgenic DNA - Quality requirements and applications
At a glance
- German title
-
Monitoring der Wirkungen gentechnisch veränderter Organismen (GVO) - Verfahren zur Extraktion von Nukleinsäuren aus Böden zur Analyse von mikrobiellen Gemeinschaften und zum Nachweis transgener DNA - Qualitätsanforderungen und Anwendungsbeispiele
- Publication date
- 2013-08
- Publisher
- Engl. VDI-Gesellschaft Technologies of Life Sciences
- Related manuals
- Number of pages
- 27
- Available in
- German, English
- Abstract
-
Extracted nucleic acids from soil can provide the source material for detecting microorganisms living in soil and can help determining their diversity. Soil nucleic acids can also be used to detect plant genes, for example transgenic DNA (recombinant genes) from genetically modified plants. The standard describes the basic principles and gives sample protocols for the extraction of nucleic acids from soil. This standard describes the quality requirements for the analysis of agricultural soils in order to determine the effects of the release or cultivation of genetically modified organisms and the associated changes in land use on the structure and function of microbial communities. Methods described also have the capacity to detect transgenic DNA (recombinant genes) entering soil through genetically modified organisms by qualitative or quantitative means.